产品货号:
GS2240
中文名称:
Bst2BI内切酶
英文名称:
Bst2BI
产品规格:
100U
发货周期:
1~3天
产品价格:
询价
储存条件:冷冻(-20℃)
使用说明
To obtain 100%activity,BSA should be added to the 1x reaction mix to a final concentration of 100μg/ml.Do not use BSA for long incubation.
技术规格
相关搜索:Bst2BI内切酶,Bst2BI限制性内切酶
使用说明
To obtain 100%activity,BSA should be added to the 1x reaction mix to a final concentration of 100μg/ml.Do not use BSA for long incubation.
技术规格
Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1hourat 60℃ in a total reaction volume of 50μl. |
Recognition sites | C↑TCGTG |
来源 | Bacillus stearothermophilus 2B |
Assayed on | Lambda DNA |
Optimal buffer | Y(33mMTris-acetate(pH 7.9 at 25℃);10 mM magnesium acetate;66 mM potassium acetate;1 mM DTT.)+ BSA |
Optimal temperature | 60℃ |
Storage conditions | 10 mM Tris-HCl(pH 7.5);200 mM NaCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;50%glycerol.Store at -20℃. |
Ligations | After 10-fold overdigestion with enzyme 95%of the DNA fragments can be ligated and recut. |
Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a.of enzyme for 16hoursat 60℃. |
Methlation effect | Methylation sensitivity not tested |
Thermal inactivation | enzyme is inactivated by incubation at 80℃ for 20 mins. |
Campatible Ends | BauI,BsiI,BssSI |
相关搜索:Bst2BI内切酶,Bst2BI限制性内切酶